Template:Cell Cycle Exercises

From Computational Cell Biology

Exercise

Work in groups of 3. Work with people from different discipline. The goal of the lab is to become familiar with the software and the process of constructing a model.

A. Draw flow diagrams or concept map for the statements provided below.

1.Create a concept map of the following:

• inactive MPF becomes active MPF
• Active MPF becomes inactive MPF

2.Concept map the following:

• Cyclin is synthesized and degraded
• Cyclin stimulates inactive MPF to become active MPF

B. Create the same concept maps in Stella. Intro to Stella Controls

C. What goes in the boxes? Reaction rates Exercise rationale

1. All reactions are linear and based on the law of mass action (rate constant x substrate).

How do we create a reaction in Stella? What are the factors in a reaction?

2. Set values for cyclin and MPF range from 0.1 to 1. Choose at least three values to examine.

3. Use two of following set of rate constants: 0.05, 0.1, 1.0

Write a notes (report) containing the following:

1. Describe the characteristics of one set of simulation results. 2. Describe briefly why you think you obtained the results you did? 3. What questions came up if any?

Exercise version 5/2/07